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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
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- Product number
- HPA001528 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA001528, RRID:AB_1078242
- Product name
- Anti-ATP5B
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human ATP5B, Gene description: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide, Alternative Gene Names: ATPSB, Validated applications: ICC, IHC, WB, Uniprot ID: P06576, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Critical roles of tubular mitochondrial ATP synthase dysfunction in maleic acid-induced acute kidney injury
Variants in ATP5F1B are associated with dominantly inherited dystonia.
miR-450a Acts as a Tumor Suppressor in Ovarian Cancer by Regulating Energy Metabolism
Evidence of Oxidative Phosphorylation in Zebrafish Photoreceptor Outer Segments at Different Larval Stages
Mesenchymal stem cells from preterm to term newborns undergo a significant switch from anaerobic glycolysis to the oxidative phosphorylation
Lin H, Liang C, Yang M, Chen P, Wang T, Chen Y, Shih Y, Liu W, Chiu C, Chiang C, Lin C, Lin H
Apoptosis 2024;29(5-6):620-634
Apoptosis 2024;29(5-6):620-634
Variants in ATP5F1B are associated with dominantly inherited dystonia.
Nasca A, Mencacci NE, Invernizzi F, Zech M, Keller Sarmiento IJ, Legati A, Frascarelli C, Bustos BI, Romito LM, Krainc D, Winkelmann J, Carecchio M, Nardocci N, Zorzi G, Prokisch H, Lubbe SJ, Garavaglia B, Ghezzi D
Brain : a journal of neurology 2023 Jul 3;146(7):2730-2738
Brain : a journal of neurology 2023 Jul 3;146(7):2730-2738
miR-450a Acts as a Tumor Suppressor in Ovarian Cancer by Regulating Energy Metabolism
Muys B, Sousa J, Plaça J, de Araújo L, Sarshad A, Anastasakis D, Wang X, Li X, de Molfetta G, Ramão A, Lal A, Vidal D, Hafner M, Silva W
Cancer Research 2019;79(13):3294-3305
Cancer Research 2019;79(13):3294-3305
Evidence of Oxidative Phosphorylation in Zebrafish Photoreceptor Outer Segments at Different Larval Stages
Calzia D, Garbarino G, Caicci F, Pestarino M, Manni L, Traverso C, Panfoli I, Candiani S
Journal of Histochemistry & Cytochemistry 2018;66(7):497-509
Journal of Histochemistry & Cytochemistry 2018;66(7):497-509
Mesenchymal stem cells from preterm to term newborns undergo a significant switch from anaerobic glycolysis to the oxidative phosphorylation
Ravera S, Podestà M, Sabatini F, Fresia C, Columbaro M, Bruno S, Fulcheri E, Ramenghi L, Frassoni F
Cellular and Molecular Life Sciences 2017;75(5):889-903
Cellular and Molecular Life Sciences 2017;75(5):889-903
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATP5B antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Sample type
- Human
- Protocol
- Protocol
