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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [4]
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- Product number
- RZ1029 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Zebrafish Atp5b Antibody / Atp5f1b / ATP synthase subunit beta
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Zebrafish
- Host
- Rabbit
- Vial size
- 100 ug
- Concentration
- Lyophilized
- Storage
- After reconstitution, the Zebrafish Atp5b antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of Atp5b/ATP5F1B protein using Zebrafish Atp5b antibody and 1) zebrafish head 2) female zebrafish viscera, 3) male zebrafish viscera, 4) whole zebrafish and 5) zebrafish embryo tissue lysate. Predicted molecular weight ~57 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE zebrafish brain tissue with Zebrafish Atp5b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE zebrafish colon tissue with Zebrafish Atp5b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE zebrafish kidney tissue with Zebrafish Atp5b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE zebrafish embryo tissue with Zebrafish Atp5b antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
