PAB28632

antibody from Abnova Corporation

Targeting: SPARC BM-40, ON, ONT

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28632

Provider

Abnova Corporation

Product name

SPARC polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant SPARC.

Antigen sequence

QEALPDETEVVEETVAEVTEVSVGANPVQVEVGEF
DDGAEETEEEVVAENPCQNHHCKHGKVCELDENNT
PMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHF
FATKCTLEGTKKGHKLHLDY


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with SPARC polyclonal antibody (Cat # PAB28632).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human esophagus with SPARC polyclonal antibody (Cat # PAB28632) shows distinct positivity in fibroblasts at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)