Antibody data
- Antibody Data
- Antigen structure
- References [39]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 15274-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15274-1-AP, RRID:AB_2194961
- Product name
- SPARC antibody
- Antibody type
- Polyclonal
- Description
- SPARC antibody (Cat. #15274-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat, pig and has been validated for the following applications: IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Modulating the tumoral SPARC content to enhance albumin-based drug delivery for cancer therapy.
FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization.
Therapeutic effects of in vivo administration of an inhibitor of tryptophan 2,3-dioxygenase (680c91) for the treatment of fibroids: a preclinical study.
SPARC in cancer-associated fibroblasts is an independent poor prognostic factor in non-metastatic triple-negative breast cancer and exhibits pro-tumor activity.
Tissue-resident CXCR4(+) macrophage as a poor prognosis signature promotes pancreatic ductal adenocarcinoma progression.
SPARC Is Highly Expressed in Young Skin and Promotes Extracellular Matrix Integrity in Fibroblasts via the TGF-β Signaling Pathway.
SPARC expression in tumor microenvironment induces partial epithelial-to-mesenchymal transition of esophageal adenocarcinoma cells via cooperating with TGF-β signaling.
Synergistic inhibition of NUDT21 by secretory S100A11 and exosomal miR-487a-5p promotes melanoma oligo- to poly-metastatic progression.
Atorvastatin reduces IOP in ocular hypertension in vivo and suppresses ECM in trabecular meshwork perhaps via FGD4.
SEC23A Inhibit Melanoma Metastatic through Secretory PF4 Cooperation with SPARC to Inhibit MAPK Signaling Pathway.
A 9-kDa matricellular SPARC fragment released by cathepsin D exhibits pro-tumor activity in the triple-negative breast cancer microenvironment.
SARS-CoV-2 Disrupts Proximal Elements in the JAK-STAT Pathway.
SPARC knockdown attenuated TGF-β1-induced fibrotic effects through Smad2/3 pathways in human pterygium fibroblasts.
The Balance between Orthodontic Force and Radiation in the Jawbone: Microstructural, Histological, and Molecular Study in a Rat Model.
Evidence for Delivery of Abraxane via a Denatured-Albumin Transport System.
Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.
The "Matrisome" reveals the characterization of skin keloid microenvironment.
MicroRNA‑592 promotes cell proliferation, migration and invasion in colorectal cancer by directly targeting SPARC.
SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.
Induction of SPARC on Oxidative Stress, Inflammatory Phenotype Transformation, and Apoptosis of Human Brain Smooth Muscle Cells Via TGF-β1-NOX4 Pathway.
Effects of transforming growth factor-β1 on plasminogen activation in stem cells from the apical papilla: role of activating receptor-like kinase 5/Smad2 and mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) signalling.
Antimetastatic Effect of Fucoidan-Sargassum against Liver Cancer Cell Invadopodia Formation via Targeting Integrin αVβ3 and Mediating αVβ3/Src/E2F1 Signaling.
Olfactory ensheathing cells facilitate neurite sprouting and outgrowth by secreting high levels of hevin.
Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration.
Effects of hydrogen peroxide on biological characteristics and osteoinductivity of decellularized and demineralized bone matrices.
Myosin-X Silencing in the Trabecular Meshwork Suggests a Role for Tunneling Nanotubes in Outflow Regulation.
Transcriptome analysis of gingival tissues of enamel-renal syndrome.
Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity.
Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.
Proteomic Analysis of Plasma Membrane Proteins of Antler Stem Cells Using Label-Free LC⁻MS/MS.
StarD13 3'-untranslated region functions as a ceRNA for TP53INP1 in prohibiting migration and invasion of breast cancer cells by regulating miR-125b activity.
miR-504 promotes tumour growth and metastasis in human osteosarcoma by targeting TP53INP1.
Mapping molecular differences and extracellular matrix gene expression in segmental outflow pathways of the human ocular trabecular meshwork.
Analysis of colorectal cancer glyco-secretome identifies laminin β-1 (LAMB1) as a potential serological biomarker for colorectal cancer.
Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.
Expression of Secreted Protein Acidic and Rich in Cysteine in the Stroma of a Colorectal Carcinoma is Associated With Patient Prognosis.
nab-Paclitaxel potentiates gemcitabine activity by reducing cytidine deaminase levels in a mouse model of pancreatic cancer.
Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer.
The pancreas cancer microenvironment.
Zheng B, Chen Y, Niu L, Zhang X, Yang Y, Wang S, Chen W, Cai Z, Huang W, Huang W
Journal of controlled release : official journal of the Controlled Release Society 2024 Feb;366:596-610
Journal of controlled release : official journal of the Controlled Release Society 2024 Feb;366:596-610
FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization.
Ma X, Zhao D, Liu S, Zuo J, Wang W, Wang F, Li Y, Ding Z, Wang J, Wang X
Oral diseases 2024 Apr;30(3):991-1003
Oral diseases 2024 Apr;30(3):991-1003
Therapeutic effects of in vivo administration of an inhibitor of tryptophan 2,3-dioxygenase (680c91) for the treatment of fibroids: a preclinical study.
Chuang TD, Ton N, Rysling S, Quintanilla D, Boos D, Khorram O
Fertility and sterility 2024 Apr;121(4):669-678
Fertility and sterility 2024 Apr;121(4):669-678
SPARC in cancer-associated fibroblasts is an independent poor prognostic factor in non-metastatic triple-negative breast cancer and exhibits pro-tumor activity.
Alcaraz LB, Mallavialle A, Mollevi C, Boissière-Michot F, Mansouri H, Simony-Lafontaine J, Laurent-Matha V, Chardès T, Jacot W, Turtoi A, Roger P, Guiu S, Liaudet-Coopman E
International journal of cancer 2023 Mar 15;152(6):1243-1258
International journal of cancer 2023 Mar 15;152(6):1243-1258
Tissue-resident CXCR4(+) macrophage as a poor prognosis signature promotes pancreatic ductal adenocarcinoma progression.
Liao Z, Ye L, Li T, Jin X, Lin X, Fei Q, Zhang H, Shi S, Yu X, Jin K, Wu W
International journal of cancer 2023 Jun 1;152(11):2396-2409
International journal of cancer 2023 Jun 1;152(11):2396-2409
SPARC Is Highly Expressed in Young Skin and Promotes Extracellular Matrix Integrity in Fibroblasts via the TGF-β Signaling Pathway.
Ham SM, Song MJ, Yoon HS, Lee DH, Chung JH, Lee ST
International journal of molecular sciences 2023 Jul 29;24(15)
International journal of molecular sciences 2023 Jul 29;24(15)
SPARC expression in tumor microenvironment induces partial epithelial-to-mesenchymal transition of esophageal adenocarcinoma cells via cooperating with TGF-β signaling.
Ma W, Yan Y, Bai S, Zhou Y, Wang X, Feng Z, Li G, Zhou S, Zhang J, Ren J
Cell biology international 2023 Jan;47(1):250-259
Cell biology international 2023 Jan;47(1):250-259
Synergistic inhibition of NUDT21 by secretory S100A11 and exosomal miR-487a-5p promotes melanoma oligo- to poly-metastatic progression.
Zeng B, Chen Y, Chen H, Zhao Q, Sun Z, Liu D, Li X, Zhang Y, Wang J, Xing HR
Molecular oncology 2023 Dec;17(12):2743-2766
Molecular oncology 2023 Dec;17(12):2743-2766
Atorvastatin reduces IOP in ocular hypertension in vivo and suppresses ECM in trabecular meshwork perhaps via FGD4.
Song XY, Chen YY, Liu WT, Cong L, Zhang JL, Zhang Y, Zhang YY
International journal of molecular medicine 2022 Jun;49(6)
International journal of molecular medicine 2022 Jun;49(6)
SEC23A Inhibit Melanoma Metastatic through Secretory PF4 Cooperation with SPARC to Inhibit MAPK Signaling Pathway.
Zeng B, Sun Z, Zhao Q, Liu D, Chen H, Li X, Xing HR, Wang J
International journal of biological sciences 2021;17(12):3000-3012
International journal of biological sciences 2021;17(12):3000-3012
A 9-kDa matricellular SPARC fragment released by cathepsin D exhibits pro-tumor activity in the triple-negative breast cancer microenvironment.
Alcaraz LB, Mallavialle A, David T, Derocq D, Delolme F, Dieryckx C, Mollevi C, Boissière-Michot F, Simony-Lafontaine J, Du Manoir S, Huesgen PF, Overall CM, Tartare-Deckert S, Jacot W, Chardès T, Guiu S, Roger P, Reinheckel T, Moali C, Liaudet-Coopman E
Theranostics 2021;11(13):6173-6192
Theranostics 2021;11(13):6173-6192
SARS-CoV-2 Disrupts Proximal Elements in the JAK-STAT Pathway.
Chen DY, Khan N, Close BJ, Goel RK, Blum B, Tavares AH, Kenney D, Conway HL, Ewoldt JK, Chitalia VC, Crossland NA, Chen CS, Kotton DN, Baker SC, Fuchs SY, Connor JH, Douam F, Emili A, Saeed M
Journal of virology 2021 Sep 9;95(19):e0086221
Journal of virology 2021 Sep 9;95(19):e0086221
SPARC knockdown attenuated TGF-β1-induced fibrotic effects through Smad2/3 pathways in human pterygium fibroblasts.
Fan J, Zhang X, Jiang Y, Chen L, Sheng M, Chen Y
Archives of biochemistry and biophysics 2021 Nov 30;713:109049
Archives of biochemistry and biophysics 2021 Nov 30;713:109049
The Balance between Orthodontic Force and Radiation in the Jawbone: Microstructural, Histological, and Molecular Study in a Rat Model.
Dorchin-Ashkenazi H, Ginat-Koton R, Gabet Y, Klein Y, Chaushu S, Dorchin H, Brosh T, Vered M
Biology 2021 Nov 18;10(11)
Biology 2021 Nov 18;10(11)
Evidence for Delivery of Abraxane via a Denatured-Albumin Transport System.
Hama M, Ishima Y, Chuang VTG, Ando H, Shimizu T, Ishida T
ACS applied materials & interfaces 2021 May 5;13(17):19736-19744
ACS applied materials & interfaces 2021 May 5;13(17):19736-19744
Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.
Morani F, Bisceglia L, Rosini G, Mutti L, Melaiu O, Landi S, Gemignani F
International journal of molecular sciences 2021 Mar 8;22(5)
International journal of molecular sciences 2021 Mar 8;22(5)
The "Matrisome" reveals the characterization of skin keloid microenvironment.
Zhang S, Liu B, Wang W, Lv L, Gao D, Chai M, Li M, Wu Z, Zhu Y, Ma J, Leng L
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Apr;35(4):e21237
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Apr;35(4):e21237
MicroRNA‑592 promotes cell proliferation, migration and invasion in colorectal cancer by directly targeting SPARC.
Pan Z, Xie R, Song W, Gao C
Molecular medicine reports 2021 Apr;23(4)
Molecular medicine reports 2021 Apr;23(4)
SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.
Chen DY, Khan N, Close BJ, Goel RK, Blum B, Tavares AH, Kenney D, Conway HL, Ewoldt JK, Kapell S, Chitalia VC, Crossland NA, Chen CS, Kotton DN, Baker SC, Connor JH, Douam F, Emili A, Saeed M
bioRxiv : the preprint server for biology 2020 Oct 28;
bioRxiv : the preprint server for biology 2020 Oct 28;
Induction of SPARC on Oxidative Stress, Inflammatory Phenotype Transformation, and Apoptosis of Human Brain Smooth Muscle Cells Via TGF-β1-NOX4 Pathway.
Tan X, Li T, Zhu S, Zhong W, Li F, Wang Y
Journal of molecular neuroscience : MN 2020 Nov;70(11):1728-1741
Journal of molecular neuroscience : MN 2020 Nov;70(11):1728-1741
Effects of transforming growth factor-β1 on plasminogen activation in stem cells from the apical papilla: role of activating receptor-like kinase 5/Smad2 and mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) signalling.
Chang MC, Chang HH, Hsieh WC, Huang WL, Lian YC, Jeng PY, Wang YL, Yeung SY, Jeng JH
International endodontic journal 2020 May;53(5):647-659
International endodontic journal 2020 May;53(5):647-659
Antimetastatic Effect of Fucoidan-Sargassum against Liver Cancer Cell Invadopodia Formation via Targeting Integrin αVβ3 and Mediating αVβ3/Src/E2F1 Signaling.
Pan TJ, Li LX, Zhang JW, Yang ZS, Shi DM, Yang YK, Wu WZ
Journal of Cancer 2019;10(20):4777-4792
Journal of Cancer 2019;10(20):4777-4792
Olfactory ensheathing cells facilitate neurite sprouting and outgrowth by secreting high levels of hevin.
Ge L, Zhuo Y, Wu P, Liu Y, Qi L, Teng X, Duan D, Chen P, Lu M
Journal of chemical neuroanatomy 2019 Nov 26;104:101728
Journal of chemical neuroanatomy 2019 Nov 26;104:101728
Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration.
Dong Z, Coates D, Liu Q, Sun H, Li C
Journal of proteomics 2019 Mar 20;195:98-113
Journal of proteomics 2019 Mar 20;195:98-113
Effects of hydrogen peroxide on biological characteristics and osteoinductivity of decellularized and demineralized bone matrices.
Qing Q, Zhang YJ, Yang JL, Ning LJ, Zhang YJ, Jiang YL, Zhang Y, Luo JC, Qin TW
Journal of biomedical materials research. Part A 2019 Jul;107(7):1476-1490
Journal of biomedical materials research. Part A 2019 Jul;107(7):1476-1490
Myosin-X Silencing in the Trabecular Meshwork Suggests a Role for Tunneling Nanotubes in Outflow Regulation.
Sun YY, Yang YF, Keller KE
Investigative ophthalmology & visual science 2019 Feb 1;60(2):843-851
Investigative ophthalmology & visual science 2019 Feb 1;60(2):843-851
Transcriptome analysis of gingival tissues of enamel-renal syndrome.
Wang YP, Lin HY, Zhong WL, Simmer JP, Wang SK
Journal of periodontal research 2019 Dec;54(6):653-661
Journal of periodontal research 2019 Dec;54(6):653-661
Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity.
Wang H, Ning T, Song C, Luo X, Xu S, Zhang X, Deng Z, Ma D, Wu B
Journal of cellular physiology 2019 Aug;234(8):12897-12909
Journal of cellular physiology 2019 Aug;234(8):12897-12909
Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.
Darville LNF, Sokolowski BHA
Proteome science 2018;16:15
Proteome science 2018;16:15
Proteomic Analysis of Plasma Membrane Proteins of Antler Stem Cells Using Label-Free LC⁻MS/MS.
Wang D, Ba H, Li C, Zhao Q, Li C
International journal of molecular sciences 2018 Nov 5;19(11)
International journal of molecular sciences 2018 Nov 5;19(11)
StarD13 3'-untranslated region functions as a ceRNA for TP53INP1 in prohibiting migration and invasion of breast cancer cells by regulating miR-125b activity.
Zheng L, Li X, Chou J, Xiang C, Guo Q, Zhang Z, Guo X, Gao L, Xing Y, Xi T
European journal of cell biology 2018 Jan;97(1):23-31
European journal of cell biology 2018 Jan;97(1):23-31
miR-504 promotes tumour growth and metastasis in human osteosarcoma by targeting TP53INP1.
Cai Q, Zeng S, Dai X, Wu J, Ma W
Oncology reports 2017 Nov;38(5):2993-3000
Oncology reports 2017 Nov;38(5):2993-3000
Mapping molecular differences and extracellular matrix gene expression in segmental outflow pathways of the human ocular trabecular meshwork.
Vranka JA, Bradley JM, Yang YF, Keller KE, Acott TS
PloS one 2015;10(3):e0122483
PloS one 2015;10(3):e0122483
Analysis of colorectal cancer glyco-secretome identifies laminin β-1 (LAMB1) as a potential serological biomarker for colorectal cancer.
Lin Q, Lim HS, Lin HL, Tan HT, Lim TK, Cheong WK, Cheah PY, Tang CL, Chow PK, Chung MC
Proteomics 2015 Nov;15(22):3905-20
Proteomics 2015 Nov;15(22):3905-20
Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.
Chen SX, Xu XE, Wang XQ, Cui SJ, Xu LL, Jiang YH, Zhang Y, Yan HB, Zhang Q, Qiao J, Yang PY, Liu F
Journal of proteomics 2014 Oct 14;110:155-71
Journal of proteomics 2014 Oct 14;110:155-71
Expression of Secreted Protein Acidic and Rich in Cysteine in the Stroma of a Colorectal Carcinoma is Associated With Patient Prognosis.
Kim JY, Jeong D, Ahn TS, Kim HJ, Park DS, Park SY, Bae SB, Lee S, Lee SS, Lee MS, Cho HD, Baek MJ
Annals of coloproctology 2013 Jun;29(3):93-9
Annals of coloproctology 2013 Jun;29(3):93-9
nab-Paclitaxel potentiates gemcitabine activity by reducing cytidine deaminase levels in a mouse model of pancreatic cancer.
Frese KK, Neesse A, Cook N, Bapiro TE, Lolkema MP, Jodrell DI, Tuveson DA
Cancer discovery 2012 Mar;2(3):260-269
Cancer discovery 2012 Mar;2(3):260-269
Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer.
Loei H, Tan HT, Lim TK, Lim KH, So JB, Yeoh KG, Chung MC
Journal of proteome research 2012 Mar 2;11(3):1759-72
Journal of proteome research 2012 Mar 2;11(3):1759-72
The pancreas cancer microenvironment.
Feig C, Gopinathan A, Neesse A, Chan DS, Cook N, Tuveson DA
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Aug 15;18(16):4266-76
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Aug 15;18(16):4266-76
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human placenta tissue were subjected to SDS PAGE followed by western blot with 15274-1-AP(SPARC antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human stomach cancer using 15274-1-AP(SPARC antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The SPARC antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SPARC. This antibody recognizes human, mouse, rat, pig antigen. The SPARC antibody has been validated for the following applications: ELISA, WB, IHC analysis.