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Western blot
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- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB942 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal SPARC Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse SPARC/Osteonectin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human SPARC is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Implication of SPARC in the modulation of the extracellular matrix and mitochondrial function in muscle cells.
SPARC Expression Did Not Predict Efficacy of nab-Paclitaxel plus Gemcitabine or Gemcitabine Alone for Metastatic Pancreatic Cancer in an Exploratory Analysis of the Phase III MPACT Trial.
Clever-1/stabilin-1 controls cancer growth and metastasis.
SPARC mediates early extracellular matrix remodeling following myocardial infarction.
Secreted protein acidic and rich in cysteine deficiency ameliorates renal inflammation and fibrosis in angiotensin hypertension.
Absence of SPARC in lens epithelial cells results in altered adhesion and extracellular matrix production in vitro.
Melouane A, Carbonell A, Yoshioka M, Puymirat J, St-Amand J
PloS one 2018;13(2):e0192714
PloS one 2018;13(2):e0192714
SPARC Expression Did Not Predict Efficacy of nab-Paclitaxel plus Gemcitabine or Gemcitabine Alone for Metastatic Pancreatic Cancer in an Exploratory Analysis of the Phase III MPACT Trial.
Hidalgo M, Plaza C, Musteanu M, Illei P, Brachmann CB, Heise C, Pierce D, Lopez-Casas PP, Menendez C, Tabernero J, Romano A, Wei X, Lopez-Rios F, Von Hoff DD
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Nov 1;21(21):4811-8
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Nov 1;21(21):4811-8
Clever-1/stabilin-1 controls cancer growth and metastasis.
Karikoski M, Marttila-Ichihara F, Elima K, Rantakari P, Hollmén M, Kelkka T, Gerke H, Huovinen V, Irjala H, Holmdahl R, Salmi M, Jalkanen S
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Dec 15;20(24):6452-64
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Dec 15;20(24):6452-64
SPARC mediates early extracellular matrix remodeling following myocardial infarction.
McCurdy SM, Dai Q, Zhang J, Zamilpa R, Ramirez TA, Dayah T, Nguyen N, Jin YF, Bradshaw AD, Lindsey ML
American journal of physiology. Heart and circulatory physiology 2011 Aug;301(2):H497-505
American journal of physiology. Heart and circulatory physiology 2011 Aug;301(2):H497-505
Secreted protein acidic and rich in cysteine deficiency ameliorates renal inflammation and fibrosis in angiotensin hypertension.
Socha MJ, Manhiani M, Said N, Imig JD, Motamed K
The American journal of pathology 2007 Oct;171(4):1104-12
The American journal of pathology 2007 Oct;171(4):1104-12
Absence of SPARC in lens epithelial cells results in altered adhesion and extracellular matrix production in vitro.
Weaver MS, Sage EH, Yan Q
Journal of cellular biochemistry 2006 Feb 1;97(2):423-32
Journal of cellular biochemistry 2006 Feb 1;97(2):423-32
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse SPARC by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line and mouse placenta tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse SPARC Monoclonal Antibody (Catalog # MAB942) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for SPARC at approximately 35-37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- SPARC/Osteonectin in Mouse Ovary. SPARC/Osteonectin was detected in perfusion fixed frozen sections of mouse ovary using Mouse SPARC/Osteonectin Monoclonal Antibody (Catalog # MAB942) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
