Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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Comment
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- Product number
- AP05261PU-N - Provider product page
- Provider
- OriGene
- Product name
- SPARC rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- SPARC rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SPARC
- Antibody clone number
- NULL
- Vial size
- 70 µg
- Concentration
- 0.29 mg/ml
Submitted references SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R
Journal of leukocyte biology 2007 Mar;81(3):748-56
Journal of leukocyte biology 2007 Mar;81(3):748-56
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: Detection of endogenous SPARC in mouse heart lysate using (0.75 ug/ml) and anti-rabbit HRP (1:75k) and using Pierce's Super Signal West Femto.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: Detection of endogenous SPARC in human hepatocellular carcinoma (HCC). 2 ug/ml , developed with Vector ImmPress anti-rabbit HRP and DAB substrate.
- Validation comment
- IHC