6678-MSM12-P1

antibody from Invitrogen Antibodies

Targeting: SPARC BM-40, ON, ONT

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Gel shift (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

6678-MSM12-P1

Provider

Invitrogen Antibodies

Product name

SPARC/Osteonectin Monoclonal Antibody (OSTN/3932)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OSTN/3932

Vial size

100 µg

Concentration

200 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of JEG-3 cell lysate using SPARC/Osteonectin Mouse Monoclonal Antibody (OSTN/3932).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human spleen stained with SPARC/Osteonectin Mouse Monoclonal Antibody (OSTN/3932).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human tonsil stained with SPARC/Osteonectin Mouse Monoclonal Antibody (OSTN/3932). Inset: PBS instead of primary antibody; secondary only negative control.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Analysis of Protein Array containing more than 19,000 full-length human proteins using SPARC/Osteonectin Mouse Monoclonal Antibody (OSTN/3932). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SDS-PAGE Analysis Purified SPARC/Osteonectin Mouse Monoclonal Antibody (OSTN/3932). Confirmation of Purity and Integrity of Antibody.