Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-80971 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-80971, RRID:AB_11035283
- Product name
- Rabbit Polyclonal SPARC Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SPARC antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Suboptimal Level of Bone-Forming Cells in Advanced Cirrhosis are Associated with Hepatic Osteodystrophy.
Expression of osteogenic molecules in the caudate nucleus and gray matter and their potential relevance for Basal Ganglia calcification in hypoparathyroidism.
Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model.
Bihari C, Lal D, Thakur M, Sukriti S, Mathur D, Patil AG, Anand L, Kumar G, Sharma S, Thapar S, Rajbongshi A, Rastogi A, Kumar A, Sarin SK
Hepatology communications 2018 Sep;2(9):1095-1110
Hepatology communications 2018 Sep;2(9):1095-1110
Expression of osteogenic molecules in the caudate nucleus and gray matter and their potential relevance for Basal Ganglia calcification in hypoparathyroidism.
Goswami R, Millo T, Mishra S, Das M, Kapoor M, Tomar N, Saha S, Roy TS, Sreenivas V
The Journal of clinical endocrinology and metabolism 2014 May;99(5):1741-8
The Journal of clinical endocrinology and metabolism 2014 May;99(5):1741-8
Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model.
Kato BS, Nicholson G, Neiman M, Rantalainen M, Holmes CC, Barrett A, Uhlén M, Nilsson P, Spector TD, Schwenk JM
Proteome science 2011 Nov 17;9:73
Proteome science 2011 Nov 17;9:73
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPARC Antibody [NBP1-80971] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPARC Antibody [NBP1-80971] - Analysis in control (vector only transfected HEK293T lysate) and SPARC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPARC Antibody [NBP1-80971] - Analysis using Anti-SPARC antibody NBP1-80971 (A) shows similar pattern to independent antibody NBP1-80972 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human endometrium shows moderate cytoplasmic positivity in stromal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human skin shows moderate cytoplasmic positivity in fibroblasts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human placenta shows moderate cytoplasmic positivity in fibroblasts.