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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- HPA003020 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA003020, RRID:AB_1079531
- Product name
- Anti-SPARC
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human SPARC, Gene description: secreted protein, acidic, cysteine-rich (osteonectin), Alternative Gene Names: ON, Validated applications: IHC, WB, Uniprot ID: P09486, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Pigment Epithelium Derived Factor Is Involved in the Late Phase of Osteosarcoma Metastasis by Increasing Extravasation and Cell-Cell Adhesion
MicroRNA‑592 promotes cell proliferation, migration and invasion in colorectal cancer by directly targeting SPARC
Levels of human proteins in plasma associated with acute paediatric malaria.
Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model
Kuriyama S, Tanaka G, Takagane K, Itoh G, Tanaka M
Frontiers in Oncology 2022;12
Frontiers in Oncology 2022;12
MicroRNA‑592 promotes cell proliferation, migration and invasion in colorectal cancer by directly targeting SPARC
Pan Z, Xie R, Song W, Gao C
Molecular Medicine Reports 2021;23(4)
Molecular Medicine Reports 2021;23(4)
Levels of human proteins in plasma associated with acute paediatric malaria.
Reuterswärd P, Bergström S, Orikiiriza J, Lindquist E, Bergström S, Andersson Svahn H, Ayoglu B, Uhlén M, Wahlgren M, Normark J, Ribacke U, Nilsson P
Malaria journal 2018 Nov 15;17(1):426
Malaria journal 2018 Nov 15;17(1):426
Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model
Kato B, Nicholson G, Neiman M, Rantalainen M, Holmes C, Barrett A, Uhlén M, Nilsson P, Spector T, Schwenk J
Proteome Science 2011;9(1):73
Proteome Science 2011;9(1):73
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and SPARC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418873).
- Sample type
- Human
- Protocol
- Protocol
