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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-27385 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Butyrylcholinesterase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: human BCHE-transfected 293T. Predicted reactivity: Mouse (87%), Rat (86%), Dog (92%), Cat (93%), Pig (94%), Chicken (81%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.27 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Discovery of Butyrylcholinesterase-Activated Near-Infrared Fluorogenic Probe for Live-Cell and In Vivo Imaging.
Liu SY, Xiong H, Yang JQ, Yang SH, Li Y, Yang WC, Yang GF
ACS sensors 2018 Oct 26;3(10):2118-2128
ACS sensors 2018 Oct 26;3(10):2118-2128
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCHE using 30 µg of 293T lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a BCHE polyclonal antibody (Product # PA5-27385) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Butyrylcholinesterase was performed by separating 30 µg of non-transfected (-) and BCHE-transfected (+) 293T whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Butyrylcholinesterase Polyclonal Antibody (Product # PA5-27385) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Butyrylcholinesterase was performed by separating 30 µg of various whole cell extracts by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a Butyrylcholinesterase Polyclonal Antibody (Product # PA5-27385) at a dilution of 1:1000. A: Jurkat, B: Raji.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SAS xenograft, using BCHE (Product # PA5-27385) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
