Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB2302 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2302, RRID:AB_1578389
- Product name
- PAK7 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PAK7.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cloning and characterization of PAK5, a novel member of mammalian p21-activated kinase-II subfamily that is predominantly expressed in brain.
p21-activated kinases: three more join the Pak.
PAK5, a new brain-specific kinase, promotes neurite outgrowth in N1E-115 cells.
Pandey A, Dan I, Kristiansen TZ, Watanabe NM, Voldby J, Kajikawa E, Khosravi-Far R, Blagoev B, Mann M
Oncogene 2002 May 30;21(24):3939-48
Oncogene 2002 May 30;21(24):3939-48
p21-activated kinases: three more join the Pak.
Jaffer ZM, Chernoff J
The international journal of biochemistry & cell biology 2002 Jul;34(7):713-7
The international journal of biochemistry & cell biology 2002 Jul;34(7):713-7
PAK5, a new brain-specific kinase, promotes neurite outgrowth in N1E-115 cells.
Dan C, Nath N, Liberto M, Minden A
Molecular and cellular biology 2002 Jan;22(2):567-77
Molecular and cellular biology 2002 Jan;22(2):567-77
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PAK7 polyclonal antibody (Cat # PAB2302) in lysates from transiently transfected COS-7 cells.Lane 1 : negative control.Lane 2 : PAK1-expressing cells.Lane 3 : PAK2-expressing cells.Lane 4 : PAK4-expressing cells.Lane 5 : PAK5-expressing cells.Lane 6 : PAK6-expressing cells.PAK5 (arrow) was detected using purified polyclonal antibody. Data is kindly provided by Drs. Z.M. Jaffer and J. Chernoff from the Fox Chase Cancer Center (Philadelphia, PA) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with PAK7 polyclonal antibody (Cat # PAB2302) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)