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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [5]
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- Product number
- PA5-51463 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NPC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PVQFKDCGSV DGVIKEVNVS PCPTQPCQLS KGQSYSVNVT FTSNIQSKSS KAVVHGILMG VPVPFPIPEP DGCKSGINCP IQKDKTYSYL NKLPVKSEYP SIKLVVEWQL QDDKNQSLFC WEIPVQIVSH Highest antigen sequence identity to the following orthologs: Mouse - 81%, Rat - 82%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NPC2 in human cell lines U2OS and HeLa using a NPC2 Polyclonal Antibody (Product # PA5-51463). Corresponding NPC2 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NPC2 in mouse cell line NIH-3T3 and rat cell line NBT-II using a NPC2 Polyclonal Antibody (Product # PA5-51463).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NPC2 in human epididymis and skeletal muscle tissues using NPC2 Polyclonal Antibody (Product # PA5-51463). Corresponding NPC2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NPC2 in human epididymis using NPC2 Polyclonal Antibody (Product # PA5-51463) shows strong granular cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NPC2 in human testis using NPC2 Polyclonal Antibody (Product # PA5-51463) shows moderate to strong granular cytoplasmic positivity in Leydig cells and cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NPC2 in human endometrium using NPC2 Polyclonal Antibody (Product # PA5-51463) shows weak granular cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NPC2 in human skeletal muscle using NPC2 Polyclonal Antibody (Product # PA5-51463) shows no positivity in myocytes as expected.
