A01582-2

antibody from Boster Biological Technology

Targeting: NPC2 EDDM1, HE1, NP-C2

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A01582-2

Provider

Boster Biological Technology

Product name

Anti-Niemann Pick C2/NPC2 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for Niemann Pick C2 detection. Tested with WB, IHC-P in Human.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Identification of pimavanserin tartrate as a potent Ca(2+)-calcineurin-NFAT pathway inhibitor for glioblastoma therapy.

Liu ZZ, Liu XN, Fan RC, Jia YP, Zhang QK, Gao XQ, Wang YQ, Yang MQ, Ji LZ, Zhou YQ, Li HL, Li P, Tang B
Acta pharmacologica Sinica 2021 Nov;42(11):1860-1874

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of Niemann Pick C2 using anti-Niemann Pick C2 antibody (A01582-2). Niemann Pick C2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Niemann Pick C2 Antibody (A01582-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image