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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- RQ4408 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NPC2 Antibody / Niemann Pick C2
- Antibody type
- Polyclonal
- Description
- This highly specific NPC2 antibody is suitable for use in Western blot/Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the NPC2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human SK-OV-3 cell lysate with NPC2 antibody at 0.5ug/ml. Predicted molecular weight: ~17 kDa, can be observed as a ~21/23 kDa doublet in human samples. (Ref 1).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human U-2 OS cell lysate with NPC2 antibody at 0.5ug/ml. Predicted molecular weight: ~17 kDa, can be observed as a ~21/23 kDa doublet in human samples. (Ref 1).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human lung cancer tissue with NPC2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human intestine cancer tissue with NPC2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human renal cancer tissue with NPC2 antibody at 2ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
