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- Product number
- A01582-3 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Niemann Pick C2/NPC2 Picoband™ Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Niemann Pick C2/NPC2 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.
Wang T, Xu J, Xu Y, Xiao J, Bi N, Gu X, Wang HL
Cell reports 2022 Mar 8;38(10):110478
Cell reports 2022 Mar 8;38(10):110478
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of NPC2 using anti-NPC2 antibody (A01582-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPC2 antigen affinity purified polyclonal antibody (Catalog # A01582-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPC2 at approximately 16-19KD. The expected band size for NPC2 is at 16KD.
- Additional image
