HPA000835

NPC2 antibody from Atlas Antibodies

EDDM1, HE1, NP-C2

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Enhanced validation

Immunohistochemistry

Enhanced validation

Provider product page for HPA000835

SELECT THIS
ANTIBODY

PERFORM KNOCKDOWN
OR KNOCKOUT

SUBMIT POSITIVE
RESULTS

GET
REWARDED

Antibody Data

Product number

HPA000835

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA000835, RRID:AB_611664

Product name

Anti-NPC2

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

PVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSY
SVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEP
DGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLV
VEWQLQDDKNQSLFCWEIPVQIVSH


Isotype

IgG

Vial size

100 µl

Storage

Store at +4¡C for short term storage. Long time storage is recommended at -20¡C.

References

Pulmonary Abnormalities in Animal Models Due to Niemann-Pick Type C1 (NPC1) or C2 (NPC2) Disease
Roszell B, Tao J, Yu K, Gao L, Huang S, Ning Y, Feinstein S, Vite C, Bates S, Buratti E
PLoS ONE 2013 July;8(7)

---

Lung Cancer Signatures in Plasma Based on Proteome Profiling of Mouse Tumor Models
Taguchi A, Politi K, Pitteri S, Lockwood W, Faça V, Kelly-Spratt K, Wong C, Zhang Q, Chin A, Park K, Goodman G, Gazdar A, Sage J, Dinulescu D, Kucherlapati R, DePinho R, Kemp C, Varmus H, Hanash S
Cancer Cell 2011 September;20(3):289-299

---

Use of narrow-range peptide IEF to improve detection of lung adenocarcinoma markers in plasma and pleural effusion
Pernemalm M, De Petris L, Eriksson H, Brandén E, Koyi H, Kanter L, Lewensohn R, Lehtiö J
PROTEOMICS 2009 July;9(13):3414-3424

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line RT-4.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Sample type

MOUSE, RAT

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines U2OS and HeLa using Anti-NPC2 antibody. Corresponding NPC2 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Show more

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human epididymis shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human endometrium shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human epididymis and endometrium tissues using Anti-NPC2 antibody. Corresponding NPC2 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more