Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- ARG55566 - Provider product page
- Provider
- Arigo
- Product name
- anti-Cathepsin D antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide from Human Cathepsin D.
- Description
- Purification with Protein A and immunogen peptide.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of HepG2, MCF-7, A431, SK-BR-3 cell line (from left to right) stained with ARG55566 anti-Cathepsin D antibody at 1:1000 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of HepG2, MCF-7, A431 cell line (from left to right) stained with ARG55566 anti-Cathepsin D antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HepG2 cells stained with ARG55566 anti-Cathepsin D antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded H.liver section stained with ARG55566 anti-Cathepsin D antibody at 1:25 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded H.breast carcinoma section stained with ARG55566 anti-Cathepsin D antibody at 1:25 dilution.