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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- H00001509-M01 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CTSD Monoclonal Antibody (3F12-1B9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: LHKFTSIRRT MSEVGGSVED LIAKGPVSKY SQAVPAVTEG PIPEVLKNYM DAQYYGEIGI GTPPQCFTVV FDTGSSNLWV PSIHCKLLDI ACWIHHKYNS DKSSTYVKNG TSFDIHYGSG SLSGYLSQDT VSVPCQSASS ASALGGVKVE RQVFGEATKQ PGITFIAAKF DGILGMAYPR ISVNNVLPVF DNLMQQKLVD QNIFSFYLSR DPDAQPGGEL MLGGTDSKYY KGSLSYLNVT RKAYWQVHLD QVEVASGLTL CKEGCEAIVD TGTSLMVGPV DEVRELQKAI GAVPLIQGEY MIPCEKVSTL PAITLKLGGK GYKLSPEDYT LKVSQAGKTL CLSGFMGMDI PPPSGPLWIL GDVFIGRYYT VFDRDNNRVG FAEAARL
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3F12-1B9
- Vial size
- 100 µg
- Concentration
- See Label
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CTSD expression in transfected 293T cell line by CTSD monoclonal antibody (M01), clone 3F12-1B9.Lane 1: CTSD transfected lysate (Predicted MW: 44.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CTSD is approximately 3 ng/mL as a capture antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to CTSD on formalin-fixed paraffin-embedded human testis. Antibody concentration is 1.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CTSD is approximately 3 ng/mL as a capture antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of CTSD transfected lysate using anti-CTSD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CTSD MaxPab rabbit polyclonal antibody.
