Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- PA3-925 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Dopamine beta Hydroxylase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA3-925 detects human, bovine, rat and mouse DBH. This antibody is not effective for immunohistochemistry in human brain. PA3-925 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~72 kDa protein representing DBH from rat neurons. In immunohistochemistry and immunofluorescence procedures, it specifically stains chromaffin cells of the adrenal medulla, and DBH containing cells of the sympathetic nervous system. The PA3-925 immunogen is a synthetic peptide corresponding to residues S(43) E P P E S P F P Y H I P L D P E G T L(62) of rat DBH. This sequence is highly conserved (>85% homology) in human, bovine and mouse.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Selective loss of catecholaminergic wake active neurons in a murine sleep apnea model.
Near simultaneous release of classical and peptide cotransmitters from chromaffin cells.
Zhu Y, Fenik P, Zhan G, Mazza E, Kelz M, Aston-Jones G, Veasey SC
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Sep 12;27(37):10060-71
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Sep 12;27(37):10060-71
Near simultaneous release of classical and peptide cotransmitters from chromaffin cells.
Whim MD
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Jun 14;26(24):6637-42
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Jun 14;26(24):6637-42
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DBH in rat neuron cells using Product # PA3-925.