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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB286-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human VEGF-D Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human VEGF-D in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) VEGF-D, rmVEGF-B, recombinant human (rh) VEGF-B and rhVEGF is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O43915- Isotype
- IgG
- Antibody clone number
- 78923
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Correlation of Vascular Endothelial Growth Factor subtypes and their receptors with melanoma progression: A next-generation Tissue Microarray (ngTMA) automated analysis.
Pulmonary Vasculopathy Associated with FIGF Gene Mutation.
Primary Tumor-Secreted Lymphangiogenic Factors Induce Pre-Metastatic Lymphvascular Niche Formation at Sentinel Lymph Nodes in Oral Squamous Cell Carcinoma.
The role of the VEGF-C/-D/flt-4 autocrine loop in the pathogenesis of salivary neoplasms.
VEGF-DdeltaNdeltaC mediated angiogenesis in skeletal muscles of diabetic WHHL rabbits.
Tbx1 regulates Vegfr3 and is required for lymphatic vessel development.
Profiling of VEGFs and VEGFRs as prognostic factors in soft tissue sarcoma: VEGFR-3 is an independent predictor of poor prognosis.
Novel vascular endothelial growth factor D variants with increased biological activity.
Prognostic significance of vascular endothelial growth factor D in breast carcinoma with long-term follow-up.
Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome.
Seyed Jafari SM, Wiedmer C, Cazzaniga S, Frangež Ž, Shafighi M, Beltraminelli H, Weber B, Simon HU, Hunger RE
PloS one 2018;13(11):e0207019
PloS one 2018;13(11):e0207019
Pulmonary Vasculopathy Associated with FIGF Gene Mutation.
Bailey E, Cui Y, Casey A, Stoler JM, Ai X, Ma D, Handin R, Sliz P, Vargas SO, El-Chemaly SY
The American journal of pathology 2017 Jan;187(1):25-32
The American journal of pathology 2017 Jan;187(1):25-32
Primary Tumor-Secreted Lymphangiogenic Factors Induce Pre-Metastatic Lymphvascular Niche Formation at Sentinel Lymph Nodes in Oral Squamous Cell Carcinoma.
Wakisaka N, Hasegawa Y, Yoshimoto S, Miura K, Shiotani A, Yokoyama J, Sugasawa M, Moriyama-Kita M, Endo K, Yoshizaki T
PloS one 2015;10(12):e0144056
PloS one 2015;10(12):e0144056
The role of the VEGF-C/-D/flt-4 autocrine loop in the pathogenesis of salivary neoplasms.
Tampouris AI, Kandiloros D, Giotakis I, Gakiopoulou H, Lazaris AC
Pathology, research and practice 2012 Mar 15;208(3):151-6
Pathology, research and practice 2012 Mar 15;208(3):151-6
VEGF-DdeltaNdeltaC mediated angiogenesis in skeletal muscles of diabetic WHHL rabbits.
Roy H, Bhardwaj S, Babu M, Lähteenvuo JE, Ylä-Herttuala S
European journal of clinical investigation 2010 May;40(5):422-32
European journal of clinical investigation 2010 May;40(5):422-32
Tbx1 regulates Vegfr3 and is required for lymphatic vessel development.
Chen L, Mupo A, Huynh T, Cioffi S, Woods M, Jin C, McKeehan W, Thompson-Snipes L, Baldini A, Illingworth E
The Journal of cell biology 2010 May 3;189(3):417-24
The Journal of cell biology 2010 May 3;189(3):417-24
Profiling of VEGFs and VEGFRs as prognostic factors in soft tissue sarcoma: VEGFR-3 is an independent predictor of poor prognosis.
Kilvaer TK, Valkov A, Sorbye S, Smeland E, Bremnes RM, Busund LT, Donnem T
PloS one 2010 Dec 14;5(12):e15368
PloS one 2010 Dec 14;5(12):e15368
Novel vascular endothelial growth factor D variants with increased biological activity.
Toivanen PI, Nieminen T, Viitanen L, Alitalo A, Roschier M, Jauhiainen S, Markkanen JE, Laitinen OH, Airenne TT, Salminen TA, Johnson MS, Airenne KJ, Ylä-Herttuala S
The Journal of biological chemistry 2009 Jun 5;284(23):16037-48
The Journal of biological chemistry 2009 Jun 5;284(23):16037-48
Prognostic significance of vascular endothelial growth factor D in breast carcinoma with long-term follow-up.
Nakamura Y, Yasuoka H, Tsujimoto M, Yang Q, Imabun S, Nakahara M, Nakao K, Nakamura M, Mori I, Kakudo K
Clinical cancer research : an official journal of the American Association for Cancer Research 2003 Feb;9(2):716-21
Clinical cancer research : an official journal of the American Association for Cancer Research 2003 Feb;9(2):716-21
Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome.
Zhou Y, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ
The American journal of pathology 2002 Apr;160(4):1405-23
The American journal of pathology 2002 Apr;160(4):1405-23
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- VEGF-D in Human Placenta. VEGF-D was detected in immersion fixed frozen sections of human placenta (cross-section through a blood vessel) using 15 µg/mL Mouse Anti-Human VEGF-D Monoclonal Antibody (Catalog # MAB286) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by VEGF-D and Neutralization by Human VEGF-D Antibody. Recombinant Human VEGF-D (Catalog # 622-VD) stimulates proliferation in HMVEC human microvascular endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF-D (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human VEGF-D Monoclonal Antibody (Catalog # MAB286). The ND50 is typically 3-8 µg/mL.
