ABIN458124

antibody from antibodies-online

Targeting: F13A1 F13A

No application information provided.

Antibody Data

Product number

ABIN458124

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN458124, RRID:AB_10780140

Product name

anti-Coagulation Factor XIII, A1 Polypeptide (F13A1) antibody

Antibody type

Polyclonal

Antigen

Plasma FXIII is an active transglutaminase until it is activated (FXIIIa). It consists of two A subunits joined as a dimer, connected to two B subunits (A2B2). Each A subunit has a molecular weight of 75,000 and each B subunit of 80,000, making an aggregate molecular weight of the whole molecule of 320,000. Normal plasma contains about 20mg/ml. Platelet FXIII consists of only A subunits synthesized in the megakaryocytes. It accounts for about 50% of the total blood FXIII activity with a linear relationship between FXIII level and platelet counts. RAHu/FXIII-A reacts with both plasma and platelet FXIII. Antiserum raised against the B subunit (RAHu/FXIII-S) reacts exclusively with native and activated FXIIIa in plasma. FXIIIa results from activation of FXIII by thrombin after the release of fibrinopeptides A from fibrinogen. Highly purified FXIII-A is used for the immunization. Freund€™s complete adjuvant is used in the first step of the immunization procedure.

Reactivity

Human

Host

Rabbit

Vial size

1 ml

Concentration

Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 when tested against appropriate concentrations of the antigen in ag

Storage

Lyophilised at +4°C (10 years), reconstituted at or below -20°C (3-5 years), reconstituted at +4°C at least 7 days