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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-22110 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Factor XIII A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299. Predicted reactivity: Mouse (86%), Rat (84%), Bovine (86%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.97 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Characterization of Ferric Chloride-Induced Arterial Thrombosis Model of Mice and the Role of Red Blood Cells in Thrombosis Acceleration.
Shim Y, Kwon I, Park Y, Lee HW, Kim J, Kim YD, Nam HS, Park S, Heo JH
Yonsei medical journal 2021 Nov;62(11):1032-1041
Yonsei medical journal 2021 Nov;62(11):1032-1041
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Factor XIIIa using 30 µg of H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Factor XIIIa polyclonal antibody (Product # PA5-22110) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Factor XIII A Polyclonal Antibody (Product # PA5-22110). Human plasma (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Factor XIII A Polyclonal Antibody (Product # PA5-22110) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Factor XIIIa in paraformaldehyde-fixed A549 cells using a Factor XIIIa polyclonal antibody (Product # PA5-22110) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A549, using Factor XIIIa antibody (Product # PA5-22110) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Factor XIII A was performed in paraffin-embedded mouse liver tissue using Factor XIII A Polyclonal Antibody (Product # PA5-22110) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Factor XIII A was performed in paraffin-embedded mouse pancreas tissue using Factor XIII A Polyclonal Antibody (Product # PA5-22110) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H520 xenograft, using Factor XIIIa (Product # PA5-22110) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 Evaluation of thrombus stability and stability-related factors by FeCl 3 concentration and mouse strain. Blood flow of the occluded carotid arteries was recorded for 2 h. Five male mice of each strain were used at each concentration. (A) Flow restoration decreased dose-dependently in both ICR and C57BL/6N mice ( p for trend=0.005, p for trend=0.001, respectively). At 10%-20%, ICR mice showed significantly lower blood flow restoration. (B) Duration of occlusion increased dose-dependently in both ICR and C57BL/6N mice ( p for trend
