ARG65714

antibody from Arigo

Targeting: VCL

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

ARG65714

Provider

Arigo

Product name

anti-Vinculin antibody

Antibody type

Polyclonal

Antigen

Recombinant protein of Human Vinculin

Description

Affinity purification with immunogen.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Storage

For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 30 µg of HeLa treated with TNFa at 0, 10, 20, and 30 min. The blots stained with ARG51850 anti-P38 MAPK phospho (Thr180/Tyr182) antibody, ARG55258 anti-p38 MAPK antibody, and ARG65714 anti-Vinculin antibody.

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 30 µg of 1) Mouse heart, and 2) HeLa cell lysate stained with ARG65714 anti-Vinculin antibody at 1:500 dilution.