HPA063777

antibody from Atlas Antibodies

Targeting: VCL

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA063777

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA063777, RRID:AB_2685121

Product name

Anti-VCL

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human VCL, Gene description: vinculin, Validated applications: WB, ICC, Uniprot ID: P18206, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

NUP133 Controls Nuclear Pore Assembly, Transcriptome Composition, and Cytoskeleton Regulation in Podocytes

Rogg M, Maier J, Ehle M, Sammarco A, Schilling O, Werner M, Schell C
Cells 2022;11(8):1259

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Murine Cytomegalovirus M25 Proteins Sequester the Tumor Suppressor Protein p53 in Nuclear Accumulations

Kutle I, Szymańska-de Wijs K, Bogdanow B, Cuvalo B, Steinbrück L, Jonjić S, Wagner K, Niedenthal R, Selbach M, Wiebusch L, Dezeljin M, Messerle M, Goodrum F
Journal of Virology 2020;94(20)

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VCL antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Sample type

Human

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to focal adhesion sites.

Sample type

Human