13-9777-82

antibody from Invitrogen Antibodies

Targeting: VCL

Provider product page for 13-9777-82

Immunocytochemistry

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [3]
Comments [0]
Validations
Immunocytochemistry [1]
Other assay [4]

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Product number: 13-9777-82 - Provider product page
Provider: Invitrogen Antibodies
Product name: Vinculin Monoclonal Antibody (7F9), Biotin, eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: Description: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression. Vinculin binds to talin, paxillin, and alpha-actinin. Disregulation of vinculin alters cell adhesion, migration, and growth, which promotes cancer invasion and metastasis. Applications Reported: This 7F9 antibody has been reported for use in microscopy, immunocytochemistry, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This 7F9 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity: Human, Mouse, Rat
Host: Mouse
Conjugate: Biotin
Isotype: IgG
Antibody clone number: 7F9
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: 4° C, store in dark, DO NOT FREEZE!

VCL protein structure - 13-9777-82 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2. Increased expression of ER-stress markers in mice with HCC. ( A ) mRNA expression of ER-stress markers Edem1, Ero1b, Grp94, Herp, Atf4, Eif2ak3, Ddit3 , and Hspa5 in liver tissue from healthy mice; and tumor tissue and surrounding non-tumoral tissue from mice with DEN-induced HCC. ( B ) Hspa5- mRNA and ( C ) protein expression of BIP in murine liver tissue. ( D ) Ratio of spliced to unspliced XBP1 in liver tissue from healthy mice; and tumor tissue and surrounding non-tumoral tissue from mice with DEN-induced HCC, treated with 4mu8C. ( E ) Representative western blot image of spliced and unspliced XBP1 protein and vinculin in healthy liver, DEN-induced HCC and DEN-induced HCC treated with 4mu8C. ( F ) quantification of spliced and unspliced XBP1, normalized to total vinculin levels. ( G ) Ratio of spliced to unspliced XBP1 protein levels. ( H ) Representative images and ( I ) quantification of liver tissue sections stained with antibodies against spliced XBP1. p-Values were calculated via the Student''s T-test with five biological replicates per group. Scale bars = 120 mum. Figure 2-figure supplement 1. Activation of the unfolded protein response is mainly located in the stroma of mice with HCC. Liver tissue from mice with DEN-induced HCC, stained with alphaSMA-antibodies and co-stained with antibodies against ( A ) spliced XBP1, ( B ) total XBP1, ( C ) IRE1alpha ( D ) phopho-IRE1alpha, and ( E ) BIP. Scale bars = 50 mum. Figure 2-figure supplement 2. Expression of
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 FA area is positively correlated with the FN concentration on substrate surface. ( a ) MEFs were seeded on the polymer coverslip-bottom mu-dishes coated with ploy-L-lysine followed by 0 to 10 mug/ml FN. After 6 hours, the cells were fixed and stained with anti-vinculin antibody for FA area determination. Scale bar, 10 mum. ( b ) The variation of FA areas in cells responding to various concentrations of FN. Data are expressed as the percentage of the total FA area of each cell relative to the cell area. Values represent mean +- standard deviation ( n = 45 in each condition.). *** P < 0.005 (Student's t-test).
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Actin polymerization is required for FA maturation. ( a ) MEFs were seeded on the 10 mug/ml FN-coated polymer coverslip-bottom mu-dishes and treated with latrunculin (La; 1.0 muM) or nocodazole (Noc; 10 muM) for 30 min followed by immunostaining with anti-vinculin antibody for FA area determination. NT, no treatment. Scale bar, 10 mum. ( b ) The variation of FA areas under various treatments. Data are expressed as the percentage of the total FA area of each cell relative to the cell area. Values represent mean +- standard deviation ( n = 45). *** P < 0.005 (Student's t-test).
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 7 Paxillin containing focal adhesion size and abundance correlates with local H 2 O 2 levels. (A) Miro1 +/+ and Miro1 -/- MEFs expressing mCherry-Paxillin and HyPer7. Red-box inserts show mCherry-Paxillin features below at areas of high and low HyPer7 oxidation levels in Miro1 +/+ MEFs and areas of low HyPer7 oxidation in Miro1 -/- MEFs. (B) Quantification of focal adhesion (FA) area (mCherry-Paxillin) at sites of high and low HyPer7 oxidation levels in Miro1 +/+ MEFs and sites of low HyPer7 oxidation in Miro1 -/- MEFs (n = average from 3-5 cells/group, *p < 0.05). (C) Quantification of the number of FAs (mCherry-Paxillin) per cell at sites of high and low HyPer7 oxidation levels in Miro1 +/+ MEFs and sitess of low HyPer7 oxidation in Miro1 -/- MEFs (n = average from 3-5 cells/group). (D) Western blot of reduced phospho-Vinculin (Y100) and phospho-p130Cas (Y410) phosphorylation status in Miro1 -/- MEFs. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.) Fig. 7
Conjugate: Biotin