Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A01207-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Vinculin/VCL Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Vinculin/VCL detection. Tested with WB, IHC-P, IF, FCM, Direct ELISA in Human;Mouse;Rat;Monkey.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Integrating network pharmacology and bioinformatics to explore and experimentally verify the regulatory effect of Buyang Huanwu decoction on glycolysis and angiogenesis after cerebral infarction.
Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice.
Inhibiting the Activity of ABCG2 by KU55933 in Colorectal Cancer.
Multilayered coating of titanium implants promotes coupled osteogenesis and angiogenesis in vitro and in vivo.
Monitoring of TGF-β 1-Induced Human Lung Adenocarcinoma A549 Cells Epithelial-Mesenchymal Transformation Process by Measuring Cell Adhesion Force with a Microfluidic Device.
Toxicity analysis of various Pluronic F-68-coated carbon nanotubes on mesenchymal stem cells.
Tian F, Yi J, Liu Y, Chen B, Wang X, Ouyang Y, Liu J, Tang Y, Long H, Liu B
Journal of ethnopharmacology 2024 Jan 30;319(Pt 3):117218
Journal of ethnopharmacology 2024 Jan 30;319(Pt 3):117218
Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice.
Behrmann A, Zhong D, Li L, Xie S, Mead M, Sabaeifard P, Goodarzi M, Lemoff A, Kozlitina J, Towler DA
Endocrinology 2023 Dec 23;165(2)
Endocrinology 2023 Dec 23;165(2)
Inhibiting the Activity of ABCG2 by KU55933 in Colorectal Cancer.
Liu K, Chen Y, Shi XB, Xing ZH, He ZJ, Wang ST, Li YC, Liu WJ, Zhang PW, Yu ZZ, Mo XM, Shi XY, Chen ZS, Shi Z
Recent patents on anti-cancer drug discovery 2022;17(4):387-395
Recent patents on anti-cancer drug discovery 2022;17(4):387-395
Multilayered coating of titanium implants promotes coupled osteogenesis and angiogenesis in vitro and in vivo.
Chen W, Xu K, Tao B, Dai L, Yu Y, Mu C, Shen X, Hu Y, He Y, Cai K
Acta biomaterialia 2018 Jul 1;74:489-504
Acta biomaterialia 2018 Jul 1;74:489-504
Monitoring of TGF-β 1-Induced Human Lung Adenocarcinoma A549 Cells Epithelial-Mesenchymal Transformation Process by Measuring Cell Adhesion Force with a Microfluidic Device.
Li Y, Gao A, Yu L
Applied biochemistry and biotechnology 2016 Jan;178(1):114-25
Applied biochemistry and biotechnology 2016 Jan;178(1):114-25
Toxicity analysis of various Pluronic F-68-coated carbon nanotubes on mesenchymal stem cells.
Yao MZ, Hu YL, Sheng XX, Lin J, Ling D, Gao JQ
Chemico-biological interactions 2016 Apr 25;250:47-58
Chemico-biological interactions 2016 Apr 25;250:47-58
No comments: Submit comment
Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of VCL using anti-VCL antibody (A01207-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human SK-OV-3 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: human SGC-7901 whole cell lysates, Lane 9: rat heart tissue lysates, Lane 10: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCL antigen affinity purified polyclonal antibody (Catalog # A01207-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCL at approximately 124KD. The expected band size for VCL is at 124KD.
- Additional image