Ab169192

antibody from Aladdin Scientific

Targeting: VCL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab169192

Provider

Aladdin Scientific

Product name

Vinculin Mouse mAb

Antibody type

Monoclonal

Description

Application:WB: 1/1,000-1/200,000 ; Predicted molecular weight: 124 kDaFlow: 0.04 _g/mL - 2.5 _g/mLICC/IF: 5.0 _g/mL

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

AB01/1F7

Vial size

100_l,10_l,1ml,20_l,50_l

Concentration

Lot by Lot

Storage

Store at +4¡C short term (1-2 weeks). Store at -20¡C or -80¡C. Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Vinculin Mouse mAb (Ab169192) - Western Blot All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 124.0 kDa Observed band size: 125.8 kDa Exposure time: 6.879 s 

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Vinculin Mouse mAb (Ab169192) - Western Blot All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL Samples: Lysate at 20 µg Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 124.0 kDa Observed band size: 122.0 kDa Exposure time: 40.1 s 

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Vinculin Mouse mAb (Ab169192) - Western Blot All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL Samples: Lysate at 20 µg Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 124.0 kDa Observed band size: 122.5 kDa Exposure time: 0.1 s 

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Vinculin Mouse mAb (Ab169192) - ICC/IF IF analysis of Vinculin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Vinculin Mouse mAb (Ab169192) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Flow cytometry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Vinculin Mouse mAb (Ab169192) - Flow Cytometry Overlay histogram showing HeLa cells stained with Vinculin Mouse mAb (Ab169192) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).