NBP1-87495

antibody from Novus Biologicals

Targeting: VCL

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-87495

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-87495, RRID:AB_11036321

Product name

Rabbit Polyclonal Vinculin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, mouse, rat Vinculin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Vinculin Antibody [NBP1-87495] - Analysis in human heart tissue.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Vinculin Antibody [NBP1-87495] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Vinculin Antibody [NBP1-87495] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VCL antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Vinculin Antibody [NBP1-87495] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Vinculin Antibody [NBP1-87495] - Staining in human smooth muscle and cerebral cortex tissues using anti-VCL antibody. Corresponding VCL RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Vinculin Antibody [NBP1-87495] - Staining of human cerebral cortex shows low expression as expected.