PA5-32639

antibody from Invitrogen Antibodies

Targeting: VCL

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-32639

Provider

Invitrogen Antibodies

Product name

Anti-Vinculin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is pancreas or small intestine tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

500 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Vinculin Polyclonal Antibody (Product # PA5-32639) and a 124kDa band corresponding to Vinculin was observed across cell lines and tissues tested. Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), NIH/3T3 (Lane 2), U-87 MG (Lane 3), PC-3 (Lane 4), MCF-7 (Lane 5), MOLT-4 (Lane 6) and Jurkat (Lane 7). Tissue extracts of Mouse Lung (Lane 8), Rat Lung (Lane 9), Mouse Brain (Lane 10) and Rat Brain (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).