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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- A303-781A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRD9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRD9 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, K-562, MCF-7, and Jurkat cells. Antibody: Affinity purified rabbit anti-BRD9 antibody (Product # A303-781A Lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRD9 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-BRD9 antibody A303-781A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. BRD9 was also immunoprecipitated by rabbit anti-BRD9 antibody BL13599, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRD9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD9 antibody (Product # A303-781A; Lot 2) used for IP at 6 µg per reaction. BRD9 was also immunoprecipitated by a previous lot of this antibody (Product # A303-781A; Lot 1) and rabbit anti-BRD9 antibody (BL13599). For blotting immunoprecipitated BRD9, Product # A303-781A-2 was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
