PAB29436

antibody from Abnova Corporation

Targeting: PCK2 PEPCK, PEPCK2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29436

Provider

Abnova Corporation

Product name

PCK2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human PCK2.

Antigen sequence

RLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGI
RDFVEHSARLCQPEGIHICDGTEAENTATLTLLEQ
QGLIRKLPKYNNCWLARTDPKD


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG sp with PCK2 polyclonal antibody (Cat # PAB29436) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line MCF7 with PCK2 polyclonal antibody (Cat # PAB29436) at 1-4 ug/mL concentration shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with PCK2 polyclonal antibody (Cat # PAB29436) shows strong cytoplasmic positivity in cells in tubules at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)