Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28078 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HepG2, Neuro2A, GL261, PC-12. Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PCK2 Polyclonal Antibody detects PCK2 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract.7.5 % SDS-PAGE. PCK2 Polyclonal Antibody (Product # PA5-28078) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PCK2 Polyclonal Antibody detects PCK2 protein by Western blot analysis. A. 30 µg HepG2 whole cell lysate/extract.7.5 % SDS-PAGE. PCK2 Polyclonal Antibody (Product # PA5-28078) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PCK2 Polyclonal Antibody detects PCK2 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract.7.5 % SDS-PAGE. PCK2 Polyclonal Antibody (Product # PA5-28078) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PCK2 Polyclonal Antibody detects PCK2 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.7.5 % SDS-PAGE. PCK2 Polyclonal Antibody (Product # PA5-28078) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of PEPCK (PCK2) was achieved by transfecting A-549 cells with PCK2 specific siRNAs (Silencer® select Product # S10125, S10126 ). Western blot analysis of PCK2 Polyclonal Antibody (Product # PA5-28078) (Fig. a) was performed using membrane enriched extracts from the knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with PCK2 Polyclonal Antibody (Product # PA5-28078, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PEPCK (PCK2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti- PCK2 Polyclonal Antibody (Product # PA5-28078) and a band around 78 kDa corresponding to PCK2 was observed in cell lines and tissues tested. Membrane enriched extracts (30 µg lysate) of A-549 (Lane 1), Neuro-2A (Lane 2), HepG2 (Lane 3), HCT 116 (Lane 4), PC-12 (Lane 5), HEK-293 (Lane 6), HeLa (Lane 7) and Mouse Small Intestine (Lane 8) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25 µg/mL, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PCK2 Polyclonal Antibody detects PCK2 protein at mitochondria by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: PCK2 protein stained by PCK2 Polyclonal Antibody (Product # PA5-28078) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using PCK2 (Product # PA5-28078) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.