PA5-28078

antibody from Invitrogen Antibodies

Targeting: PCK2 PEPCK, PEPCK2

Antibody data

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Validation data

Product number: PA5-28078 - Provider product page
Provider: Invitrogen Antibodies
Product name: PCK2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Recommended positive controls: HepG2, Neuro2A, GL261, PC-12. Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

PCK2 protein structure - PA5-28078 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: PCK2 Polyclonal Antibody detects PCK2 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract.7.5 % SDS-PAGE. PCK2 Polyclonal Antibody (Product # PA5-28078) dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: PCK2 Polyclonal Antibody detects PCK2 protein by Western blot analysis. A. 30 µg HepG2 whole cell lysate/extract.7.5 % SDS-PAGE. PCK2 Polyclonal Antibody (Product # PA5-28078) dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: PCK2 Polyclonal Antibody detects PCK2 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract.7.5 % SDS-PAGE. PCK2 Polyclonal Antibody (Product # PA5-28078) dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: PCK2 Polyclonal Antibody detects PCK2 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.7.5 % SDS-PAGE. PCK2 Polyclonal Antibody (Product # PA5-28078) dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of PEPCK (PCK2) was achieved by transfecting A-549 cells with PCK2 specific siRNAs (Silencer® select Product # S10125, S10126 ). Western blot analysis of PCK2 Polyclonal Antibody (Product # PA5-28078) (Fig. a) was performed using membrane enriched extracts from the knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with PCK2 Polyclonal Antibody (Product # PA5-28078, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PEPCK (PCK2).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti- PCK2 Polyclonal Antibody (Product # PA5-28078) and a band around 78 kDa corresponding to PCK2 was observed in cell lines and tissues tested. Membrane enriched extracts (30 µg lysate) of A-549 (Lane 1), Neuro-2A (Lane 2), HepG2 (Lane 3), HCT 116 (Lane 4), PC-12 (Lane 5), HEK-293 (Lane 6), HeLa (Lane 7) and Mouse Small Intestine (Lane 8) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25 µg/mL, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).