Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NB100-2422 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2422, RRID:AB_10001284
- Product name
- Goat Polyclonal PCK2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise isoform 1 (NP_004554) only, no cross-reactivity with PCK1.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Downregulation of miR-181a upregulates sirtuin-1 (SIRT1) and improves hepatic insulin sensitivity.
Expression of phosphoenolpyruvate carboxykinase gene in human adipose tissue: induction by rosiglitazone and genetic analyses of the adipocyte-specific region of the promoter in type 2 diabetes.
Expression of phosphoenolpyruvate carboxykinase gene in human adipose tissue: induction by rosiglitazone and genetic analyses of the adipocyte-specific region of the promoter in type 2 diabetes.
Zhou B, Li C, Qi W, Zhang Y, Zhang F, Wu JX, Hu YN, Wu DM, Liu Y, Yan TT, Jing Q, Liu MF, Zhai QW
Diabetologia 2012 Jul;55(7):2032-43
Diabetologia 2012 Jul;55(7):2032-43
Expression of phosphoenolpyruvate carboxykinase gene in human adipose tissue: induction by rosiglitazone and genetic analyses of the adipocyte-specific region of the promoter in type 2 diabetes.
Duplus E, Benelli C, Reis AF, Fouque F, Velho G, Forest C
Biochimie 2003 Dec;85(12):1257-64
Biochimie 2003 Dec;85(12):1257-64
Expression of phosphoenolpyruvate carboxykinase gene in human adipose tissue: induction by rosiglitazone and genetic analyses of the adipocyte-specific region of the promoter in type 2 diabetes.
Duplus E, Benelli C, Reis AF, Fouque F, Velho G, Forest C
Biochimie 2003 Dec;85(12):1257-64
Biochimie 2003 Dec;85(12):1257-64
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: PCK2 Antibody [NB100-2422] - Staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.