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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- PA5-40073 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZBTB38 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Nuclear extracts of HeLa cells (40 µg) were analyzed by Western Blot using a ZBTB38 polyclonal antibody (Product # PA5-40073) at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 138 kDa) is indicated on the right; the marker (kDa) is shown on the left.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- To determine the titer, an ELISA was performed using a serial dilution of the anti-human ZBTB38 antibody (Product # PA5-40073). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:26,000.
