TA501704

antibody from OriGene

Targeting: RIT2 RIBA, RIN

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501704

Provider

OriGene

Proper citation

OriGene Cat#TA501704, RRID:AB_11125705

Product name

Anti-RIT2 mouse monoclonal antibody, clone OTI4B5 (formerly 4B5)

Antibody type

Monoclonal

Description

Anti-RIT2 mouse monoclonal antibody, clone OTI4B5 (formerly 4B5)

Host

Mouse

Conjugate

Unconjugated

Epitope

RIT2

Isotype

IgG

Antibody clone number

OTI4B5

Vial size

100 µl

Concentration

0.84 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIT2 (RC205367, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIT2.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RIT2 monoclonal antibody.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC205367 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RIT2 antibody(TA501704), and then analyzed by flow cytometry.

Validation comment

FC