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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ7854 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- RIT2 Antibody / Ras-like protein expressed in neurons
- Antibody type
- Polyclonal
- Description
- This highly specific RIT2 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the RIT2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human SH-SY5Y, 2) human SK-N-SH, 3) U-87 MG, 4) U-251, 5) rat brain and 6) mouse brain tissue lysate with RIT2 antibody. Predicted molecular weight: 17-25 kDa (multiple isoforms).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-2 OS cells with RIT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RIT2 antibody.
