NBP1-31733
antibody from Novus Biologicals
Targeting: MYH9
DFNA17, EPSTS, FTNS, MHA, NMHC-II-A, NMMHCA
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP1-31733 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31733, RRID:AB_2147312
- Product name
- Rabbit Polyclonal non-muscle Myosin IIA Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - A. 30 ug NIH-3T3 whole cell lysate/extract B. 30 ug BCL-1 whole cell lysate/extract C. 30 ug Raw264.7 whole cell lysate/extract D. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - A. 30 ug Rat2 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1-2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: non-muscle Myosin IIA Antibody [NBP1-31733] - Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 ug of MYH9 antibody [N1-2], N-term. Western blot analysis was performed using MYH9 antibody [N1-2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-31733] - Paraffin-embedded human breast carcinoma. MYH9 antibody [N1-2], N-term diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-31733] - Paraffin-embedded mouse prostate. MYH9 antibody [N1-2], N-term diluted at 1:400.