PA5-27506

antibody from Invitrogen Antibodies

Targeting: MYH9 DFNA17, EPSTS, FTNS, MHA, NMHC-II-A, NMMHCA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27506

Provider

Invitrogen Antibodies

Product name

MYH9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, NT2D1, PC-3.

Concentration

0.55 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MYH9 Polyclonal Antibody detects MYH9 protein by Western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract. B. 30 µg PC-3 whole cell lysate/extract.5 % SDS-PAGE. MYH9 Polyclonal Antibody (Product # PA5-27506) dilution: 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MYH9 Polyclonal Antibody detects MYH9 protein by Western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract. B. 30 µg PC-3 whole cell lysate/extract.5 % SDS-PAGE. MYH9 Polyclonal Antibody (Product # PA5-27506) dilution: 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MYH9 Polyclonal Antibody detects MYH9 protein by Western blot analysis. A. 30 µg U87-MG whole cell lysate/extract. B. 30 µg SK-N-SH whole cell lysate/extract. C. 30 µg IMR32 whole cell lysate/extract. D. 30 µg SK-N-AS whole cell lysate/extract.5 % SDS-PAGE. MYH9 Polyclonal Antibody (Product # PA5-27506) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MYH9 Polyclonal Antibody detects MYH9 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MYH9 stained by MYH9 Polyclonal Antibody (Product # PA5-27506) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using MYH9 (Product # PA5-27506) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of MYH9 was performed in HeLa whole cell extracts using 5 µg of MYH9 Polyclonal Antibody (Product # PA5-27506). Samples were transferred to a membrane and probed with MYH9 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.