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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- MA5-27754 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HBZ Monoclonal Antibody (GT1544)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: K562 Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GT1544
- Vial size
- 100 µL
- Concentration
- 1.54 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Hemoglobin zeta in whole cell lysate using 30 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with Hemoglobin zeta monoclonal antibody (Product # MA5-27754) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HBZ Monoclonal Antibody (GT1544) (Product # MA5-27754). Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with HBZ Monoclonal Antibody (GT1544) (Product # MA5-27754) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HBZ Monoclonal Antibody (GT1544) detects Hemoglobin zeta protein at cytoplasm by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hemoglobin zeta protein stained by HBZ Monoclonal Antibody (GT1544) (Product # MA5-27754) diluted at 1:500. Blue: Hoechst 33342 staining.
