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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- R32748 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Hemoglobin Antibody (Subunit alpha)
- Antibody type
- Polyclonal
- Description
- This highly specific Hemoglobin antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Hemoglobin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat spleen and 2) human U-2 OS cell lysate with Hemoglobin antibody at 0.5ug/ml. Predicted molecular weight ~15 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human placental tissue lysate with Hemoglobin antibody. Predicted molecular weight ~15 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human renal cancer tissue with Hemoglobin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human liver cancer tissue with Hemoglobin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human placental tissue with Hemoglobin antibody. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil tissue with Hemoglobin antibody. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human K562 cells with Hemoglobin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Hemoglobin antibody.
