Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP17352PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP17352PU-N, RRID:AB_10658702
- Product name
- anti ETV4 / PEA3 (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 426~455 amino acids from the C-terminal region of Human ETV4.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- (LEFT) Western blot analysis of ETV4 antibody (C-term) Cat.-No AP17352PU-N in mouse heart tissue lysates (35 µg/lane). ETV4 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of ETV4 (arrow) using ETV4 Antibody (C-term) Cat.-No AP17352PU-N. 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ETV4 gene (Lane 2) .
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with ETV4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of widr cells using ETV4 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.