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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- F52486 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SP1 Antibody
- Antibody type
- Monoclonal
- Antigen
- This SP1 antibody was produced from a mouse immunized with recombinant Specificity Protein 1.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 1326CT463.109.176
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the SP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from (1) HeLa and (2) Ramos cell line using SP1 antibody at 1:1000. Reported molecular weight: 81-95 kDa
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent image of HeLa cells stained with SP1 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using SP1 antibody (green) and an isotype control of mouse IgG1 (blue); Ab was diluted at 1:25 dilution.
