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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00006667-M05 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006667-M05, RRID:AB_875843
- Product name
- SP1 monoclonal antibody (M05), clone 4B11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant SP1.
- Antigen sequence
QLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIAN
APGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDA
QPQAGRRTRREACTCPYCKDSEGRGSG*- Isotype
- IgG
- Antibody clone number
- 4B11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SP1 expression in transfected 293T cell line by SP1 monoclonal antibody (M05), clone 4B11.Lane 1: SP1 transfected lysate(80.693 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SP1 monoclonal antibody (M05), clone 4B11. Western Blot analysis of SP1 expression in Raw 264.7(Cat # L024V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SP1 is approximately 10ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
