Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- Ab128849 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant SP1 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human SP1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.Application:WB: 1/500-1/1000IHC: 1/50-1/100IF/ICC:1/50-1/200Protein Function:Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 554-568
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant SP1 Antibody (Ab128849) - Western Blot All lanes: Recombinant SP1 Antibody (Ab128849) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 81 kDa Observed band size: 89 kDa Exposure time: 93.7 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant SP1 Antibody (Ab128849) - IF/ICC Immunocytochemistry analysis of SP1 (green) in HeLa using Recombinant SP1 Antibody (Ab128849), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant SP1 Antibody (Ab128849) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using Recombinant SP1 Antibody (Ab128849). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.