PAB21249

FITM1 antibody from Abnova Corporation

FIT1

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for PAB21249

Antibody Data

Product number

PAB21249

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21249, RRID:AB_10968265

Product name

FIT1 polyclonal antibody

Provider product page

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant FIT1.

Antigen sequence

RRVAVTARHLSRLVVGAAVWRGAGRAFLLIEDLTG
SCFEPLPQGLLLHELPDRRSCLAAGHQWRGYTVSS
HTF


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with FIT1 polyclonal antibody (Cat # PAB21249).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with FIT1 polyclonal antibody (Cat # PAB21249) shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts and leydig cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)