PA5-41797

antibody from Invitrogen Antibodies

Targeting: ZRSR2 U2AF1-RS2, U2AF1L2, URP, ZC3H22

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-41797

Provider

Invitrogen Antibodies

Product name

ZRSR2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: HHDDYYSRLR GRRNPSPDHS YKRNGESERK SSRHRGKKSH KRTSKSRERH Sequence homology: Cow: 83%; Dog: 92%; Guinea Pig: 100%; Horse: 79%; Human: 100%; Mouse: 90%; Rabbit: 100%; Rat: 93%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Zrsr2 and functional U12-dependent spliceosome are necessary for follicular development.

Gómez-Redondo I, Pericuesta E, Navarrete-Lopez P, Ramos-Ibeas P, Planells B, Fonseca-Balvís N, Vaquero-Rey A, Fernández-González R, Laguna-Barraza R, Horiuchi K, Gutiérrez-Adán A
iScience 2022 Feb 18;25(2):103860

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Diagrams of Zrsr2 gene structure and mutations generated in this study and their expression (A) Diagram of the Zrsr2 gene with its functional domains: ZF1 and ZF2 (zinc finger domains 1 and 2), RRM (RNA recognition motif), and RS (arginine-serine rich). The red lightning indicates the target region for each different mutation. The protein sizes and structures produced by the different mutations ( Zrsr2 muA , Zrsr2 muB , and Zrsr2 muC ) are represented in the lower panels, shaded in pink. The position of the primers used for Figure 1 C (2-3F and 7R) and Figure 1 C (9F and 11R) are indicated. (B) Western blot analysis of ZRSR2 protein expression in spleen of WT and the three mutant mice, using ACTB as a loading control. The positions of molecular mass markers are indicated on the left. Blue arrows indicate ZRSR2 mutant protein produced by different alternative splicing events. (C) Expression level of Zrsr2 mRNA in ovaries from 3-month-old females of the three mutant lines and WT mice determined by RT-qPCR. Biological triplicate data for qPCR are presented as mean +- SEM. Bars with different superscripts differ significantly (p < 0.05). (D) Non-quantitative RT-PCR analysis of Zrsr2 in WT and Zrsr2 muA , Zrsr2 muB , and Zrsr2 muC mice. The right panel shows a diagram of the affected exons in Zrsr2 muC mice. Isoforms were confirmed by sequencing.