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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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- Product number
- PA5-52247 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ELF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MTSAVVDSGG TILELSSNGV ENQEESEKVS EYPAVIVEPV PSARLEQGYA AQVLVYDDET YMMQDVAEEQ EVETENVETV EASVHSSNAH CTDKTIEAAE ALLHMESPTC LRDSRSPEFI HAAMRPDVIT ETVVEVSTEE SEP
- Concentration
- 0.1 mg/mL
Submitted references Topologically Associated Domains Delineate Susceptibility to Somatic Hypermutation.
Senigl F, Maman Y, Dinesh RK, Alinikula J, Seth RB, Pecnova L, Omer AD, Rao SSP, Weisz D, Buerstedde JM, Aiden EL, Casellas R, Hejnar J, Schatz DG
Cell reports 2019 Dec 17;29(12):3902-3915.e8
Cell reports 2019 Dec 17;29(12):3902-3915.e8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ELF2 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an ELF2 Polyclonal Antibody (Product # PA5-52247).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ELF2 in Lane 1: Marker (kDa) 229, 112, 84, 48, 32, 27, 17; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using an ELF2 Polyclonal Antibody (Product # PA5-52247).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ELF2 in human cell line U-251 MG shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using an ELF2 Polyclonal Antibody (Product # PA5-52247).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ELF2 in human cell line U-2 OS using an ELF2 Polyclonal Antibody (Product # PA5-52247) shows localization to nucleoplasm, nuclear bodies and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ELF2 in human colon, lymph node, placenta and testis using ELF2 Polyclonal Antibody (Product # PA5-52247) (A) shows similar protein distribution across tissues to an independent ELF2 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ELF2 in human testis using ELF2 Polyclonal Antibody (Product # PA5-52247).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ELF2 in human lymph node using ELF2 Polyclonal Antibody (Product # PA5-52247).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ELF2 in human colon using ELF2 Polyclonal Antibody (Product # PA5-52247).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ELF2 in human placenta using ELF2 Polyclonal Antibody (Product # PA5-52247).
