PA5-52247

antibody from Invitrogen Antibodies

Targeting: ELF2 EU32, NERF, NERF-1A, NERF-1B, NERF-2

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52247

Provider

Invitrogen Antibodies

Product name

ELF2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Immunogen sequence: MTSAVVDSGG TILELSSNGV ENQEESEKVS EYPAVIVEPV PSARLEQGYA AQVLVYDDET YMMQDVAEEQ EVETENVETV EASVHSSNAH CTDKTIEAAE ALLHMESPTC LRDSRSPEFI HAAMRPDVIT ETVVEVSTEE SEP Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 85%.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Topologically Associated Domains Delineate Susceptibility to Somatic Hypermutation.

Senigl F, Maman Y, Dinesh RK, Alinikula J, Seth RB, Pecnova L, Omer AD, Rao SSP, Weisz D, Buerstedde JM, Aiden EL, Casellas R, Hejnar J, Schatz DG
Cell reports 2019 Dec 17;29(12):3902-3915.e8

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of ELF2 in human cell line U-251 MG shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using an ELF2 Polyclonal Antibody (Product # PA5-52247).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of ELF2 in human cell line U-2 OS using an ELF2 Polyclonal Antibody (Product # PA5-52247) shows localization to nucleoplasm, nuclear bodies and cytosol.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ELF2 in human colon, lymph node, placenta and testis using ELF2 Polyclonal Antibody (Product # PA5-52247) (A) shows similar protein distribution across tissues to an independent ELF2 Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ELF2 in human lymph node using ELF2 Polyclonal Antibody (Product # PA5-52247).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ELF2 in human placenta using ELF2 Polyclonal Antibody (Product # PA5-52247).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ELF2 in human testis using ELF2 Polyclonal Antibody (Product # PA5-52247).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ELF2 in human colon using ELF2 Polyclonal Antibody (Product # PA5-52247).