AF2944

antibody from Novus Biologicals

Targeting: ZBTB16 PLZF, ZNF145

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2944

Provider

Novus Biologicals

Product name

Goat Polyclonal PLZF Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human PLZF in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

PLZF(pos)c-KIT(pos)-delineated A(1)-A(4)-differentiating spermatogonia by subset and stage detection upon Bouin fixation.

Tang RL, Fan LQ
Asian journal of andrology 2019 May-Jun;21(3):309-318

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Biological and RNA regulatory function of MOV10 in mammalian germ cells.

Fu K, Tian S, Tan H, Wang C, Wang H, Wang M, Wang Y, Chen Z, Wang Y, Yue Q, Xu Q, Zhang S, Li H, Xie J, Lin M, Luo M, Chen F, Ye L, Zheng K
BMC biology 2019 May 14;17(1):39

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EXOSC10/Rrp6 is post-translationally regulated in male germ cells and controls the onset of spermatogenesis.

Jamin SP, Petit FG, Kervarrec C, Smagulova F, Illner D, Scherthan H, Primig M
Scientific reports 2017 Nov 8;7(1):15065

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DDX4-EGFP transgenic rat model for the study of germline development and spermatogenesis.

Gassei K, Sheng Y, Fayomi A, Mital P, Sukhwani M, Lin CC, Peters KA, Althouse A, Valli H, Orwig KE
Biology of reproduction 2017 Mar 1;96(3):707-719

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The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells.

Beyaz S, Kim JH, Pinello L, Xifaras ME, Hu Y, Huang J, Kerenyi MA, Das PP, Barnitz RA, Herault A, Dogum R, Haining WN, Yilmaz ÖH, Passegue E, Yuan GC, Orkin SH, Winau F
Nature immunology 2017 Feb;18(2):184-195

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Multiple layers of transcriptional regulation by PLZF in NKT-cell development.

Mao AP, Constantinides MG, Mathew R, Zuo Z, Chen X, Weirauch MT, Bendelac A
Proceedings of the National Academy of Sciences of the United States of America 2016 Jul 5;113(27):7602-7

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CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo.

Li M, Huang R, Jiang X, Chen Y, Zhang Z, Zhang X, Liang P, Zhan S, Cao S, Songyang Z, Huang J
PloS one 2015;10(11):e0143148

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Reconstruction of mouse testicular cellular microenvironments in long-term seminiferous tubule culture.

Mäkelä JA, Toppari J, Rivero-Müller A, Ventelä S
PloS one 2014;9(3):e90088

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PAX7 expression defines germline stem cells in the adult testis.

Aloisio GM, Nakada Y, Saatcioglu HD, Peña CG, Baker MD, Tarnawa ED, Mukherjee J, Manjunath H, Bugde A, Sengupta AL, Amatruda JF, Cuevas I, Hamra FK, Castrillon DH
The Journal of clinical investigation 2014 Sep;124(9):3929-44

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PLZF mediates the PTEN/AKT/FOXO3a signaling in suppression of prostate tumorigenesis.

Cao J, Zhu S, Zhou W, Li J, Liu C, Xuan H, Yan J, Zheng L, Zhou L, Yu J, Chen G, Huang Y, Yu Z, Feng L
PloS one 2013;8(12):e77922

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BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs.

Mathew R, Seiler MP, Scanlon ST, Mao AP, Constantinides MG, Bertozzi-Villa C, Singer JD, Bendelac A
Nature 2012 Nov 22;491(7425):618-21

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human PLZF by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HEL 92.1.7 human erythroleukemic cell line, LNCaP human prostate cancer cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PLZF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2944) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PLZF at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human PLZF by Simple Western^TM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for PLZF at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PLZF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2944) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.