IC83951R-100UG

antibody from R&D Systems

Targeting: ZBTB16 PLZF, ZNF145

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC83951R-100UG

Provider

R&D Systems

Product name

Mouse PLZF Alexa Fluor® 647-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse PLZF in direct ELISAs.

Reactivity

Mouse

Host

Rat

Conjugate

Red dye

Antigen sequence

NP_001028496


Isotype

IgG

Antibody clone number

816421

Vial size

100 ug

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of PLZF in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes stimulated with Recombinant Mouse IL-12 (Catalog # 419-ML) and Recombinant Mouse IL-18 (Catalog # 9139-IL) for 3 days were stained with Rat Anti-Mouse NKp46/NCR1 PE-conjugated Monoclonal Antibody (Catalog # FAB22252P) and either (A) Rat Anti-Mouse PLZF Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC83951R) or (B) Rat IgG_2A Alexa Fluor 647 Isotype Control (Catalog # IC006R). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.