17-9322-80

antibody from Invitrogen Antibodies

Targeting: ZBTB16 PLZF, ZNF145

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

17-9322-80

Provider

Invitrogen Antibodies

Product name

Anti-PLZF Monoclonal Antibody (9E12), APC, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

This 9E12 monoclonal antibody reacts with human and mouse promyelocytic leukemia zinc finger (PLZF), a member of the BTB-POZ family of transcription factors. Expression of this transcriptional repressor in immune cells differs between mice and humans. In mice, PLZF is highly expressed in immature CD1d-resricted NKT2 and NKT1 cells, and a subset of gamma delta (Vg1.1+Vd6.3+) T cells. Studies have also demonstrated expression of PLZF in non-invariant CD1d-restricted T cells, as well as non-CD1d-restricted innate T cells. In humans, PLZF is expressed in NK cells, gamma delta T cells, as well as CD4 and CD8+ T cells. PLZF is also expressed in MR1-specific mucosal-associated invariant T cells, as well as in MHC Class II-restricted T cells that develop via a thymocyte-thymocyte interaction in humans. PLZF is expressed by innate lymphoid cells and its progenitor cells. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells, NK cell function, cellular quiescence, growth suppression, and maintenance of homeostatis. Finally, PLZF has been shown to inhibit gene expression induced by retinoic acid receptor. This 9E12 antibody stains mouse thymocytes brighter than Mags.21F7. This 9E12 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human, Mouse

Isotype

IgG

Antibody clone number

9E12

Vial size

25 µg

Concentration

0.2 mg/mL

Storage

4° C, store in dark, DO NOT FREEZE!

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LEFT: C57BL/6 thymocytes were surface stained with Anti-Mouse NK1.1 PE-Cyanine7 (Product # 25-5941-82) and Anti-Mouse TCR beta FITC (Product # 11-5961-82) followed by intracellular staining with 0.06 µg of Anti-Human/Mouse PLZF APC using the Foxp3/Transcription Factor Staining Buffer Set and protocol (Product # 00-5523-00). Viable, TCR beta+ cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis. RIGHT: Normal human peripheral blood cells were surface stained with Anti-Human CD56 (NCAM) FITC (Product # 11-0566-42) followed by intracellular staining with 0.06 µg of Anti-Human/Mouse PLZF APC using the Foxp3/Transcription Factor Staining Buffer Set and protocol (Product # 00-5523-00). Cells in the lymphocyte gate were used for analysis.